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Part:BBa_K763000:Design

Designed by: Pedro Luis Dorado Morales   Group: iGEM12_Valencia_Biocampus   (2012-09-03)

pLac + Gene encoding AmCyan


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design considerations needed.

Source

The sequence of the promoter comes from E. coli genome, whereas the sequence encoding the AmCyan fluorescent protein is not natural, since it was optimized for codon usage and maximum fluorescence production.

References

1. F. Jacob and J. Monod. (1959) Genes of structure and genes of regulation in the biosynthesis of proteins. C. R. Hebd. Seances Acad. Sci.249: 1282–4.

2. S., Busby and R.H., Ebright. (2001). Transcription activation by catabolite activator protein (CAP). J. Mol. Biol.293: 199–213.

3. RE., Huber, K., Wallenfels and G.,Kurz. (1975)The action of beta-galactosidase (Escherichia coli) on allolactose.Canadian Journal of Biochemistry, 53(9):1035-1038